Journal: Scientific Reports

Article Title: Bacterial bioburden and community structure of potable water used in the International Space Station

doi: 10.1038/s41598-022-19320-3

Figure Lengend Snippet: Bacterial community structure in the PWD water determined by 16S rRNA gene amplicons sequenced on Illumina MiSeq, iSeq, or Nanopore MinION.

Article Snippet: A 16S rRNA sequencing library was constructed from 10 µL of extracted DNA using the 16S barcoding kit (Oxford Nanopore Technologies).

Techniques:

Journal: Scientific Reports

Article Title: Bacterial bioburden and community structure of potable water used in the International Space Station

doi: 10.1038/s41598-022-19320-3

Figure Lengend Snippet: Relative abundance of bacterial species in the PWD water determined by 16S rRNA gene targeted amplicon sequencing with the MinION sequencer. Genus and species with an abundance >1% are presented.

Article Snippet: A 16S rRNA sequencing library was constructed from 10 µL of extracted DNA using the 16S barcoding kit (Oxford Nanopore Technologies).

Techniques: Amplification, Sequencing

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Standardization of 16S rRNA gene sequencing using nanopore long read sequencing technology for clinical diagnosis of culture negative infections

doi: 10.3389/fcimb.2025.1517208

Figure Lengend Snippet: Average relative abundance (%) of bacterial organisms present in MCM2α and MCM2β obtained by ONT sequencing using the R10.4.1 MinION flow cell and ONT 16S Barcoding all-in-one kit (v14). Three concentrations of the MCM2α and MCM2β materials were tested in triplicate (neat, 1:10 and 1:100) and analysis by EPI2ME Desktop agent. The species abundance (%) of each dilution is reported, including the dPCR reported composition for the two materials ( Supplementary Data 3 ).

Article Snippet: Normalized extracts were amplified and barcoded using the ONT 16S Barcoding all-in-one kit (Oxford Nanopore, SQK-16S024) as per manufacturer's instructions (maximum cycle number of 25 cycles).

Techniques: Sequencing

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Standardization of 16S rRNA gene sequencing using nanopore long read sequencing technology for clinical diagnosis of culture negative infections

doi: 10.3389/fcimb.2025.1517208

Figure Lengend Snippet: Average relative abundance (%) of bacterial organisms present in MCM2α and MCM2β obtained by ONT sequencing using the R10.4.1 MinION flow cell and the in-house developed 16S ONT RBK method. Three concentrations of the MCM2α and MCM2β materials were tested in triplicate (neat, 1:10 and 1:100) and amplified in two PCRs targeting the V1-V2 and V1-V9 genomic regions of the 16S rRNA gene; analysis by EPI2ME Desktop agent. The species abundance (%) of each dilution is reported, including the dPCR reported composition for the two materials ( Supplementary data 3 ).

Article Snippet: Normalized extracts were amplified and barcoded using the ONT 16S Barcoding all-in-one kit (Oxford Nanopore, SQK-16S024) as per manufacturer's instructions (maximum cycle number of 25 cycles).

Techniques: Sequencing, Amplification